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Molecular characterization of Fasciola jacksoni from wild elephants (Elephas maximus maximus) of Sri Lanka: a taxonomic evaluation

Published online by Cambridge University Press:  24 June 2019

R. P. V. J. Rajapakse ,
S. P. Lawton Open the ORCID record for S. P. Lawton [Opens in a new window] ,
K. J. K. Karunathilake ,
B. V. P. Perera ,
N. T. B. Nguyen  and
T. H. Le
R. P. V. J. Rajapakse
Affiliation:
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka
S. P. Lawton*
Affiliation:
Molecular Parasitology Laboratory, School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Kingston Upon Thames, Surrey, KT12 EE, 32, UK
K. J. K. Karunathilake
Affiliation:
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka
B. V. P. Perera
Affiliation:
Department of Wildlife Conservation, Elephant Trust Home, Udawalave 70190, Sri Lanka
N. T. B. Nguyen
Affiliation:
Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd., Cau Giay, Hanoi, Vietnam
T. H. Le
Affiliation:
Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd., Cau Giay, Hanoi, Vietnam Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd., Cau Giay, Hanoi, Vietnam
*
Author for correspondence: S. P. Lawton, E-mail: s.p.lawton@kingston.ac.uk
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Abstract

Fasciola jacksoni is a significant contributor to the health and mortality of Asian elephants, particularly those in Sri Lanka. Despite the impact of fascioliasis on elephant populations, it is a neglected veterinary disease with limited taxonomic understanding. Molecular characterization and phylogenetic analysis of F. jacksoni were carried out to evaluate its suggested basal position in the Fasciolidae. Adult worms were collected during post-mortem of elephants, and eggs were collected from living elephants in National parks across Sri Lanka. Using the mitochondrial genes nicotinamide dehydrogenase subunit 1 (nad1) and cytochrome oxidase subunit 1 (cox1), and a partial 28S ribosomal DNA (28S rDNA), DNA sequences were generated from the F. jacksoni adult and egg material. Maximum likelihood (ML) phylogenetic analyses did not resolve F. jacksoni to be basal to the Fasciolidae. Furthermore, the ML analyses showed that the genus Fasciola was not monophyletic and that F. jacksoni was a sister species to the deer liver fluke Fascioloides magna. A clear framework is required to determine the taxonomic status of F. jacksoni and this current study provides the first detailed application of molecular techniques from multiple hosts across Sri Lanka with the production of reference DNA sequences for this important parasite.

Keywords

Asian elephantsFasciola jacksonifasciolidaemitochondrial genesphylogenetic analysisrDNA sequencesSri Lanka

Information

Type
Research Article
Information
Parasitology , Volume 146 , Issue 10 , September 2019 , pp. 1247 - 1255
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Copyright
Copyright © Cambridge University Press 2019 

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References

Alahakoon, J (1994) Presence of liver fluke Fasciola jacksoni in a Sri Lankan elephant. Gajah 12, 46–47.Google Scholar
Bhalerao, GD (1933) The trematode parasites of the Indian elephant, Elephas indicus. Indian Journal of Veterinary Science and Animal Husbandry 3, 103–155.Google Scholar
Caple, IW, Jainudeen, MR, Buick, TD and Song, CY (1978) Some clinico-pathologic findings in elephants (Elephas maximus) infected with Fasciola jacksoni. Journal of Wildlife Diseases 14, 110–115.Google Scholar
Cobbold, T. S. (1869). Fasciola jacksoni. Quarterly Journal of Microscopical Science; Supplement to Entozoa. p. 80.Google Scholar
Cobbold, TS (1882) VII. The parasites of elephants. Transactions of the Linnean Society of London. 2nd Series: Zoology 2, 223–258.Google Scholar
Fernando, P (2000) Elephants in Sri Lanka: past present and future. Loris 22, 38–44.Google Scholar
Fernando, P, Wikramanayake, E, Weerakoon, D, Jayasinghe, L, Gunawardene, M and Janaka, H (2005) Perceptions and patterns of human–elephant conflict in old and new settlements in Sri Lanka: insights for mitigation and management. Biodiversity and Conservation 14, 2465–2481.Google Scholar
Heneberg, P (2013) Phylogenetic data suggest the reclassification of Fasciola jacksoni (Digenea: Fasciolidae) as Fascioloides jacksoni comb. nov. Parasitology Research 112, 1679–1689.Google Scholar
Hing, S, Othman, N, Nathan, S, Fox, M, Fisher, M and Goossens, B (2013) First parasitological survey of Endangered Bornean elephants Elephas maximus borneensis. Endangered Species Research 21, 223–230.Google Scholar
Hing, S, Narayan, EJ, Thompson, RCA and Godfrey, SS (2016) The relationship between physiological stress and wildlife disease: consequences for health and conservation. Wildlife Research 43, 51.Google Scholar
Islam, S (1997) Studies on some aspects of fascioliasis in Asian elephants (Elephas maximus). Journal of Veterinary Parasitology 11, 109.Google Scholar
Králová-Hromadová, I, Špakulová, M, Horáčková, E, Turčeková, L, Novobilský, A, Beck, R, Koudela, B, Marinculić, A, Rajský, D and Pybus, M (2008) Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. Journal of Parasitology 94, 58–67.Google Scholar
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870–1874.Google Scholar
Le, TH, De, NV, Agatsuma, T, Nguyen, TGT, Nguyen, QD, Mcmanus, DP and Blair, D (2008) Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola hepatica and Fasciola gigantica in Vietnam. International Journal for Parasitology 38, 725–730.Google Scholar
Le, TH, Nguyen, KT, Nguyen, NTB, Doan, HTT, Dung, DT and Blair, D (2017) The ribosomal transcription units of Haplorchis pumilio and H. taichui and the use of 28S rDNA sequences for phylogenetic identification of common heterophyids in Vietnam. Parasites & Vectors 10. doi: 10.1186/s13071-017-1968-0.Google Scholar
Liu, G-H, Gasser, RB, Young, ND, Song, H-Q, Ai, L and Zhu, X-Q (2014) Complete mitochondrial genomes of the ‘intermediate form’ of Fasciola and Fasciola gigantica, and their comparison with F. hepatica. Parasites & Vectors 7, 150.Google Scholar
Lorimer, J (2010) Elephants as companion species: the lively biogeographies of Asian elephant conservation in Sri Lanka. Transactions of the Institute of British Geographers 35, 491–506.Google Scholar
Lotfy, WM, Brant, SV, DeJong, RJ, Le, TH, Demiaszkiewicz, A, Rajapakse, RPVJ, Perera, VBVP, Laursen, JR and Loker, ES (2008) Evolutionary origins, diversification, and biogeography of liver flukes (Digenea, Fasciolidae). American Journal of Tropical Medicine and Hygiene 79, 248–255.Google Scholar
Mas-Coma, S, Valero, MA and Bargues, MD (2009) Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Advances in Parasitology 69, 41–146.Google Scholar
Mucheka, VT, Lamb, JM, Pfukenyi, DM and Mukaratirwa, S (2015) DNA sequence analyses reveal co-occurrence of novel haplotypes of Fasciola gigantica with F. hepatica in South Africa and Zimbabwe. Veterinary Parasitology 214, 144–151.Google Scholar
Nguyen, TGT, De, NV, Vercruysse, J, Dorny, P and Le, TH (2009) Genotypic characterization and species identification of Fasciola spp. with implications regarding the isolates infecting goats in Vietnam. Experimental Parasitology 123, 354–361.Google Scholar
Nolan, MJ and Cribb, TH (2005) The use and implications of ribosomal DNA sequencing for the discrimination of digenean species. Advances in Parasitology 60, 101–163.Google Scholar
Perera, BVP and Rajapakse, RPVJ (2009). Mortality and morbidity of wild elephants (Elephas maximus maximus) of Sri Lanka, as a result of liver flukes (Fasciola jacksoni) infestation. In Wibbelt, G, Kretschmar, P, Hofer, H and Seet, S (eds), Proceedings of the International Conference on Diseases of Zoo and Wild Animals. Leibniz Institute for Zoo and Wildlife Research, Beekse Bergen, The Netherlands, 20–24 May 2009, p. 191.Google Scholar
Perera, BVP, Rajapakse, RPVJ, Silva-fletcher, A, Thewarage, LD and Jinadasa, HRN (2015) Emerging parasitic and infectious diseases of wild Asian Elephants (Elephas Maximus) in Udawalawe National Park, Sri Lanka. American Association of Zoo Veterinarians. Oregon, USA.Google Scholar
Singh, KP, Srivastava, VK, Prasad, A and Pandey, AP (1994) Pathology due to Fasciola jacksoni in Indian elephants (Elephas indicus). Indian Journal of Animal Science 648, 802–804.Google Scholar
Tkach, VV, Kudlai, O and Kostadinova, A (2016) Molecular phylogeny and systematics of the Echinostomatoidea Looss, 1899 (Platyhelminthes: Digenea). International Journal for Parasitology 46, 171–185.Google Scholar
Wey-Fabrizius, AR, Podsiadlowski, L, Herlyn, H and Hankeln, T (2013) Platyzoan mitochondrial genomes. Molecular Phylogenetics and Evolution 69, 365–375.Google Scholar
Windsor, R and Scott, W (1976) Fascioliasis and Salmonellosis in African elephants in captivity. British Veterinary Journal 132, 313–317.Google Scholar
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