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The maintenance of laboratory colonies of Culicoides variipennis (Coq.), C. nubeculosus (Mg.) and C. riethi Kieff. (Diptera, Ceratopogonidae)

Published online by Cambridge University Press:  10 July 2009

J. Boorman
J. Boorman
Affiliation:
Animal Virus Research Institute, Pirbright, Working, Surrey GU24 0NF, England
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Abstract

A method is described by which Culicoides variipennis (Coq.), C. riethi Kieff. and C. nubeculosus (Mg.) have been maintained as laboratory colonies for over five years. Techniques are given for the maintenance of adults, larvae and pupae; with some modifications these could probably be applied to a range of other species. Adult midges were held in waxed card pill boxes, and provided with sugar solution. Anaesthetised mice provided a blood-meal source. Larvae were reared in pans on a substrate of glass fibre in a medium which contained nutrient broth, grass meal and Bemax. The life-cycle of these species occupies 10–21 days at 23–25°C.

Information

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 64 , Issue 3 , November 1974 , pp. 371 - 377
Copyright
Copyright © Cambridge University Press 1974

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References

Boorman, J. & Gibbs, E. P. J. (1973). Multiplication of the virus of epizootic haemorrhagic disease of deer in Culicoides species (Diptera, Ceratopogonidae).—Arch. ges. Virusforsch. 41, 259–266.CrossRefGoogle ScholarPubMed
Downes, J. A. (1950). Habits and life-cycle of Culicoides nubeculosus Mg.—Nature, Lond. 166, 510–511.CrossRefGoogle ScholarPubMed
Hair, J. A. & Turner, E. C. Jr. (1966). Laboratory colonization and mass-production procedures for Culicoides guttipennis.—Mosquito News 26, 429–433.Google Scholar
Hill, M. A. (1947). The life-cycle and habits of Culicoides impunctatus Goetghebuer and Culicoides obsoletus Meigen, together with some observations on the life-cycle of Culicoides odibilis Austen, Culicoides pallidicornis Kieffer, Culicoides cubitalis Edwards and Culicoides chiopterus Meigen.—Ann. trop. Med. Parasit. 41, 55–115.CrossRefGoogle Scholar
Jones, R. H. (1957). The laboratory colonization of Culicoides variipennis (Coq.).—J. econ. Ent. 50, 107–108.CrossRefGoogle Scholar
Jones, R. H. (1960). Mass-production methods for the colonization of Culicoides variipennis sonorensis.—J. econ. Ent. 53, 731–735.CrossRefGoogle Scholar
Jones, R. H. & Foster, N. M. (1966). The transmission of bluetongue virus to embryonating chicken eggs by Culicoides variipennis (Diptera, Ceratopogonidae) infected by intrathoracic inoculation.—Mosquito News 26, 185–189.Google Scholar
Jones, R. H., Potter, H. W. Jr. & Baker, S. K. (1969). An improved larval medium for colonized Culicoides variipennis.—J. econ. Ent. 62, 1483–1486.CrossRefGoogle Scholar
Linley, J. R. (1968). Colonisation of Culicoides furens.—Ann. ent. Soc. Am. 61, 1486–1490.CrossRefGoogle ScholarPubMed
Megahed, M. M. (1956). A culture method for Culicoides nubeculosus (Meigen) (Diptera: Ceratopogonidae) in the laboratory, with notes on the biology.—Bull. ent. Res. 47, 107–114.CrossRefGoogle Scholar
Mellor, P. S. (1971). A membrane feeding technique for infection of Culicoides nubeculosus Mg. and Culicoides variipennis sonorensis Coq. with Onchocerca cervicalis Rail, and Henry.—Trans. R. Soc. trop. Med. Hyg. 65, 199–201.CrossRefGoogle Scholar
Sun, W. K. C. (1969). laboratory colonization of two biting midges, Culicoides arakawae (Arakawa) and C. schultzei (Enderlein) (Diptera, Ceratopogonidae).—Biol. Bull., Dep. Biol, Coll. Sci., Tunghai Univ. no. 39, 8 pp.Google Scholar