This study discusses the production of Eco-friendly mask using mushroom-based pulps through physicochemical treatment of mushrooms. Ultimately, it explores an approach to reduce the usage of petrochemical materials. Through the treatment of fruiting bodies from two mushroom species, Pleurotus ostreatus (Oyster mushroom) and Flammulina velutipes (Enoki mushroom), pulp was produced. Then, it was used in wet-laid sheet processing to fabricate both pure MBP sheets and composites blended with cotton. The manufactured textile was subjected to various property analyses, including antibacterial test and was also used to produce a mask prototype. In conclusion, although improvements in breathability are currently needed for practical application, the potential for further research is vast and promising. This study contributes to the advancement of sustainable, biodegradable materials as a solution to environmental challenges posed by the widespread use of synthetic polymers.