The development of externalizing behavior in young children is shaped by the complex interaction of temperament, neural mechanisms, and environmental factors. This study explored how child frontal alpha asymmetry (FAA) and child negative affect jointly moderate the relationship between mindful parenting and child externalizing behavior. The sample, drawn from families in the Netherlands, included reports from 128 mothers and 103 partners on mindful parenting, and on children’s negative affect and externalizing behavior. FAA was measured in 95 four-year-old children during an EEG session while they watched an animated video. Results indicated that children with high negative affect and greater left-sided FAA displayed the most externalizing behavior when maternal mindful parenting was low, but the least when mindful parenting was high. In contrast, no significant effects were found for children with lower negative affect or in partner-reported data. These findings suggest that children with both high negative affect and greater left-sided FAA are more sensitive to the quality of mindful parenting, particularly from mothers, aligning with the environmental sensitivity framework. Future research should replicate these findings, ideally in a larger sample, and further examine the long-term, cumulative impact of FAA and negative affect on the development of behavioral problems.

The role of the gut microbiome in infant development has gained increasing interest in recent years. Most research on this topic has focused on the first three to four years of life because this is a critical period for developing gut-brain connections. Prior studies have identified associations between the composition and diversity of the gut microbiome in infancy and markers of temperament, including negative affect. However, the specific microbes affected, and the directionality of these associations have differed between studies, likely due to differences in the developmental period of focus and assessment approaches. In the current preregistered study, we examined connections between the gut microbiome, assessed at two time points in infancy (2 weeks and 18 months), and negative affect measured at 30 months of age in a longitudinal study of infants and their caregivers. We found that infants with higher gut microbiome diversity at 2 weeks showed more observed negative affect during a study visit at 30 months. We also found evidence for associations between specific genera of bacteria in infancy and negative affect. These results suggest associations between specific features of the gut microbiome and child behavior may differ based on timing of gut microbiome measurement.

While research on leadership and employee physical ill-being is burgeoning, the short- and long-term mechanisms through which leadership influences employee physical ill-being remain underexplored. This research, grounded in leadership theories and the Job Demand-Resource (JD-R) theory, examines how transformational and abusive leadership behaviors influence employee physical ill-being through two conflict-related negative motivational mechanisms (negative work–home interactions and job role conflict) and two negative affective mechanisms representing short-term (negative affect) and long-term (burnout) mechanisms. Employing a three-wave longitudinal design over 6 months (N = 234), our findings from a multilevel path analysis revealed that transformational and abusive leadership had respectful, negative and positive effects on employee physical ill-being via conflict-related negative motivational mechanisms and short- and long-term affective mechanisms. Notably, the influence of leadership behaviors on employee physical ill-being was more pronounced through the short-term affective mechanism (negative affect) than the long-term affective mechanism (burnout). Our findings provide a nuanced understanding of how leadership behaviors affect employee physical ill-being over time, shedding light on the dynamic interplay of motivational and affective pathways in this relationship.

This study relied on the affective events theory and the social exchange theory to develop a framework that explains how situational factors (daily micro-interruptions) enhance affective reactions (negative affect) and, in turn, impair health conditions (mental health) at work. We further delineate theoretical arguments to propose the pet-human’s health effect by demonstrating that pets are boundary conditions that attenuate this relation, and as such are protective conditions for employees’ mental health. We conducted a 5-day diary study with two groups of participants, one with participants who owned pets (N = 82 x 5 = 410), and the other who did not own pets (N = 87 x 5 = 435). The multilevel results showed an indirect effect of daily micro-interruptions on individuals’ mental health through negative affect, with a daily backdrop of poorer mental health for those who did not own a pet (compared to those who owned a pet). These results evidence the benefits of owning a pet for individuals’ mental health, even at work, and as such provide recommendations for teleworking practices. Moreover, this study resorts to an innovative and robust data collection method to demonstrate the pet-human’ health effect. This study expands knowledge on the role of pets in working daily routines and shows that pets may be a personal resource for individuals while working.

The pubertal transition is characterized by pronounced sex hormone fluctuation, refinement of affective neural circuitry, and an increased risk of depression in female adolescents. Sex hormones, including testosterone, exert modulatory effects on frontal-limbic brain networks and are associated with emotion dysregulation and depressive symptoms. Weekly changes in hormones predict affective symptoms in peripubertal female adolescents, particularly in the context of stress; however, the biobehavioral mechanisms underlying hormone change and mood relationships during the pubertal transition have yet to be determined and was the objective of the present study. Forty-three peripubertal female adolescents (ages 11–14) collected 8-weekly salivary hormone (estrone, testosterone) samples and mood assessments to evaluate hormone-mood relationships, followed by a biobehavioral testing session with psychosocial stress and EEG. Within-person correlations between weekly hormone changes and corresponding mood were performed to determine individual differences in mood sensitivity to weekly hormone change. Increased frontal theta activity indexing emotion reactivity, reduced cortisol reactivity, and reduced vagal efficiency predicted the strength of the relationship between testosterone and mood. Further, testosterone-sensitivity strength was associated with the enhancement of negative affect following stress testing. Results identify divergent frontal theta and stress responses as potential biobehavioral mechanisms underlying mood sensitivity to peripubertal testosterone fluctuation.

Personality traits, automatic thoughts, and affective states during sexual activity in men have been studied; however, little is known about their interaction. The current study examines the moderation role of personality traits on the relationship between cognitive-affective dimensions and sexual behavior in men. An online sample of 497 men (227 gay men) was recruited, and participants completed a sociodemographic questionnaire, the NEO-Five Factor Inventory (NEO-FFI), the Automatic Thoughts from the Sexual Modes Questionnaire (SMQ) subscale, The Positive Affect-Negative Affect scales (PANAS), and the International Index of Erectile Function (IIEF)/and the IIEF for men who have sex with men (IIEF-MSM). The main findings showed that extraversion, absence of erotic thoughts, positive affect, and negative affect were significant predictors of sexual functioning in gay (β = .266, β = –.345, β = .361; β = –.292, p < .05, respectively) and heterosexual men (β = .208, β = –.382, β = .318; β = –.214, p < .05, respectively); neuroticism significantly predicted sexual functioning only in gay men (β = –.244, p < .05). Extraversion was a moderator between absence of erotic thoughts and sexual functioning in heterosexual men (p = .004), and between positive affect and sexual functioning in gay men (p = .001), and Neuroticism was a moderator between positive affect and sexual functioning in gay men (p < .001). Overall, extraversion buffered the negative impact of absence of erotic thoughts on heterosexual men sexual functioning and the negative impact of lower positive affect on gay men sexual functioning, whereas low neuroticism boost the impact of positive affect on gay men sexual functioning.

Mortality rates are often used in population-level animal welfare assessments because they are assumed to reflect rates of disease or injury and other problems likely to cause poor welfare. High mortality is thus assumed to correlate with factors likely to cause negative affective states. Here, we argue that negative affective states are also related to mortality rates more directly, via causal rather than merely correlational routes. In humans, negative affective states predict elevated morbidity and mortality rates as well as decreased longevity, while self-reported happiness does the opposite. This review investigates whether mortality rates and longevity can thus be used to make inferences regarding past affective states in animals. The proposed mechanism is that chronic stressors cause negative affective states and thence harmful physiological consequences through continual activation of the hypothalamic-pituitary-adrenal and sympathetic-adreno-medullary axes, which in turn can lead to increased mortality. The convergent validity of mortality as an indicator of past negative affect is demonstrated via examples of how stressors such as bereavement in humans and social isolation in social non-human species cause negative affective states, and then increase the morbidity of potentially lethal conditions such as cardiovascular disease, cancer and HIV/AIDS, ultimately leading to increased mortality and reduced longevity in both humans and animals. The potential drawbacks of using mortality rates to infer animal welfare are also discussed, including its low sensitivity and the multiplicity of factors unrelated to affective states that can influence mortality rates. However, providing these issues are accounted for, it is suggested that this indicator has value in welfare assessment, and is especially well suited for cases where animals are allowed to live out their natural lifespans, such as tends to be the case with zoo and companion animals.

Attention biases to threat are considered part of the etiology of anxiety disorders. Attention bias variability (ABV) quantifies intraindividual fluctuations in attention biases and may better capture the relation between attention biases and psychopathology risk versus mean levels of attention bias. ABV to threat has been associated with attentional control and emotion regulation, which may impact how caregivers interact with their child. In a relatively diverse sample of infants (50% White, 50.7% female), we asked how caregiver ABV to threat related to trajectories of infant negative affect across the first 2 years of life. Families were part of a multi-site longitudinal study, and data were collected from 4 to 24 months of age. Multilevel modeling examined the effect of average caregiver attention biases on changes in negative affect. We found a significant interaction between infant age and caregiver ABV to threat. Probing this interaction revealed that infants of caregivers with high ABV showed decreases in negative affect over time, while infants of caregivers with low-to-average ABV showed potentiated increases in negative affect. We discuss how both high and extreme patterns of ABV may relate to deviations in developmental trajectories.

Transactional processes between parental support and adolescents’ depressive symptoms might differ in the short term versus long term. Therefore, this multi-sample study tested bidirectional within-family associations between perceived parental support and depressive symptoms in adolescents with datasets with varying measurement intervals: Daily (N = 244, Mage = 13.8 years, 38% male), bi-weekly (N = 256, Mage = 14.4 years, 29% male), three-monthly (N = 245, Mage = 13.9 years, 38% male), annual (N = 1,664, Mage = 11.1 years, 51% male), and biennial (N = 502, Mage = 13.8 years, 48% male). Preregistered random-intercept cross-lagged panel models (RI-CLPMs) showed negative between- and within-family correlations. Moreover, although the preregistered models showed no within-family lagged effect from perceived parental support to adolescent depressive symptoms at any timescale, an exploratory model demonstrated a negative lagged effect at a biennial timescale with the annual dataset. Concerning the reverse within-family lagged effect, increases in adolescent depressive symptoms predicted decreases in perceived parental support 2 weeks and 3 months later (relationship erosion effect). Most cross-lagged effects were not moderated by adolescent sex or neuroticism trait level. Thus, the findings mostly support adolescent-driven effects at understudied timescales and illustrate that within-family lagged effects do not generalize across timescales.