Introduction. Perseaamericana (avocado) fruit is known to be a rich sourceof proteins and minerals, as well as vitamins. Although many biologicalactivities have been reported for avocado fruit, far less attentionhas been paid to the biological properties of its lipid-solublebioactive substances. The aim of this study was to evaluate theantioxidant and antihemolytic activity of lipid-soluble bioactivecompounds of avocado fruit. Materials and methods.Antioxidant and antihemolytic activity of lipid-soluble bioactivecompounds of avocado fruit was assessed by employing different methods.Also, the ability of avocado fruit to protect fluorescein againstoxidant-induced bleaching was evaluated. Results and discussion.The tested sample showed good antioxidant and antihemolytic activities. Therewas no significant difference between the reducing power of lipid-soluble bioactivesubstances in avocado fruits and ascorbic acid (p <0.05). The tested sample showed weak ferrous ion-chelating activity.The sample exhibited moderate hydrogen peroxide scavenging activity, butexhibited good antioxidant activity. Avocado fruit showed very good activityagainst 2,2′-azobis(2-amidinopropane) dihydrochloride- and hydrogenperoxide-induced hemolysis in erythrocytes. Furthermore, it showed goodprotective effects against peroxyl radical-induced bleaching in fluorescein. Conclusion. Lipid-soluble bioactive substances of avocado fruitshowed good activity in all of the studied models.