The aggregate backscattering cross-section, σ ag is the sum of backscattering cross-sections of all fish in a shoal. It is a basic acoustical parameter used for shoal description and biomass estimation. Simulations were undertaken for evaluating the impact of horizontal dimension, density, depth and beam width on measurements of σ ag for shoals with constant abundance. The important factor determining measurement bias is the ratio of shoal size expressed as along cross-section length relative to along ship beam width at mean shoal depth. The results show that 10 log10(σ ag) is underestimated by about 8 dB for a 5 m long shoal located at 200 m depth if detected by a 7° beam. A formula for correcting σ ag estimates for shoal sizes bigger than 1.3 times the beam width is proposed. The negative measurement bias can also be reduced by using transducers with narrower beam widths.