The history and practice of party polarization in Congress is a gendered concept. Men have comprised the overwhelming majority of legislators from both parties, served as their party’s leaders, and dominated the party caucuses. As women and women of color have increased their presence in the institution, particularly among Democrats, gender and race have emerged as important themes in understanding party polarization in contemporary congresses. In an analysis of legislative activity of members in the 104th to the 117th Congresses, I find the two most distinct groups of partisans, Democratic women and Republican men, are prominently featured in the opposing party’s negative messaging to constituents and voters. The prominence of Democratic women as the focal point of negative messaging from the opposition has significant consequences for this group of officeholders. This study enhances our understanding of how gender dynamics inform party polarization in legislatures.