This study investigated the hypothesis that 9- to 11-month-old multilingual infants learning Advanced Tongue Root (ATR) harmony languages (such as Akan) alongside other non-vowel harmony languages in Ghana (Africa) can use ATR harmony cues for speech segmentation. Using the central fixation procedure, infants were familiarized with bisyllabic words in two passages, one with ATR cues and one without, and then tested on isolated familiarized and novel bisyllabic words. Results indicate that, as a group, infants segmented words in their native language using ATR harmony cues, showing a familiarity preference. No effect of exposure to ATR harmony language(s) was found. These results provide the first evidence of word segmentation in infants learning between two and five languages, and with infants in Africa. The findings contribute to our understanding of multilingual infants’ language processing, suggesting their sensitivity to phonotactic cues for speech processing.