The COVID-19 pandemic revealed the fractured state of global health law infrastructure. Establishing a One Health framework in law and policy is necessary to address the multitude of interlinked global health and sustainability challenges, including the risk of emerging and re-emerging infectious diseases, climate change, antimicrobial resistance, and food insecurity. This chapter will look at domestic and regional institutional collaborative frameworks focused on One Health, drawing on the development and implementation of integrated frameworks at the country level including, Egypt, Vietnam, Kenya, and India. Additionally, it will see how regional cooperation in the Arctic has led to the adoption and implementation of One Health policy guidelines and frameworks at the domestic level. The examination of national approaches will provide a critical analysis of key opportunities and barriers for domestic policy guidelines moving forward.