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Resveratrol conjugated with silica nanoparticles used for in vitro bovine oocytes maturation

Published online by Cambridge University Press:  07 July 2025

Soraia Rage Rezende Open the ORCID record for Soraia Rage Rezende [Opens in a new window] ,
Mayara Mafra Soares ,
Graciele Freitas Cardoso ,
Deize de Cássia Antonino Rissato ,
Giovanna Faria de Moraes ,
Gustavo Pereira Cadima ,
Kele Amaral Alves ,
Benner Geraldo Alves ,
Tatiane Moraes Arantes  and
Rodrigo Otavio Decaria de Salles Rossi Open the ORCID record for Rodrigo Otavio Decaria de Salles Rossi [Opens in a new window]
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Soraia Rage Rezende
Affiliation:
Universidade Federal de Uberlândia, Uberlandia, MG, Brazil
Mayara Mafra Soares
Affiliation:
Universidade Federal de Uberlândia, Uberlandia, MG, Brazil
Graciele Freitas Cardoso
Affiliation:
Universidade Federal de Uberlândia, Uberlandia, MG, Brazil
Deize de Cássia Antonino Rissato
Affiliation:
Universidade de São Paulo Faculdade de Medicina de Ribeirão Preto, Ribeirao Preto, SP, Brazil
Giovanna Faria de Moraes
Affiliation:
Universidade Federal de Uberlândia, Uberlandia, MG, Brazil
Gustavo Pereira Cadima
Affiliation:
Universidade Federal de Uberlândia, Uberlandia, MG, Brazil
Kele Amaral Alves
Affiliation:
Pacific BioLabs Inc, Hercules, CA, USA
Benner Geraldo Alves
Affiliation:
Lander Veterinary Clinic Inc, Turlock, CA, USA
Tatiane Moraes Arantes
Affiliation:
Universidade Federal de Jataí, Jatai, GO, Brazil
Rodrigo Otavio Decaria de Salles Rossi
Affiliation:
Instituto Federal do Triângulo Mineiro, Uberlandia, MG, Brazil
Ricarda Maria dos Santos*
Affiliation:
Universidade Federal de Uberlândia, Uberlandia, MG, Brazil
*
Corresponding author: Ricarda Maria dos Santos; Email: ricarda.santos@ufu.br
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Abstract

The objective was to evaluate the use of resveratrol conjugated with silica nanoparticles during the in vitro maturation of bovine oocytes. The oocytes were divided into the following treatment groups during the maturation process: control (n = 159), resveratrol 0.5 μM (n = 158), resveratrol 1 μM (n = 155), nanoparticles conjugated with 0.5 μM resveratrol (n = 159), and nanoparticles conjugated with 1 μM resveratrol (n = 158). Several parameters were assessed, including cumulus oophorus size, reactive oxygen species (ROS) production, oocyte nuclear maturation, cell apoptosis, cleavage rates, and blastocyst production rates. Statistical analysis was conducted using Sigma Plot software (version 11) and SAS Studio, with statistical significance defined as P ≤ 0.05 for the main effects and interactions. The results indicated that the cumulus oophorus size was smaller in the resveratrol 1 μM treatment group, and the oocyte size was reduced in the nanoparticle 1 μM treatment group. No significant differences were detected between the treatment groups in terms of ROS production, oocyte maturation, or cell apoptosis. However, the resveratrol 1 μM treatment group exhibited decreased rates of cleavage and blastocyst formation. In contrast, the nanoparticles 0.5 μM and 1.0 μM treatments showed improved cleavage and blastocyst rates compared with the resveratrol 1.0 μM treatment group. In summary, while resveratrol alone at 1 μM concentration had a negative impact on cleavage and blastocyst rates, the use of silica nanoparticles conjugated with resveratrol (both 0.5 μM and 1 μM) enhanced these outcomes, suggesting a potential advantage in using nanoparticle-conjugated resveratrol for the in vitro maturation of bovine oocytes.

Keywords

Antioxidantsembryosin vitro embryos productionoxidative stressreactive oxygen species

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Type
Research Article
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Zygote , First View , pp. 1 - 6
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© The Author(s), 2025. Published by Cambridge University Press

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