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In mouse oocytes the mitochondrion-originated germinal body-like structures accumulate mouse Vasa homologue (MVH) protein

Published online by Cambridge University Press:  29 April 2014

Arkadiy A. Reunov  and
Yulia A. Reunova
Arkadiy A. Reunov*
Affiliation:
Laboratory of Cell Differentiation, Paltchevsky st.-17, A.V. Zhirmunsky Institute of Marine Biology, Far East Branch of the Russian Academy of Sciences, 690041 Vladivostok, Russia.
Yulia A. Reunova
Affiliation:
A.V. Zhirmunsky Institute of Marine Biology FEB RAS, Laboratory of Cell Differentiation, Paltchevsky st.-17, Vladivostok 690041, Russia.
*
All correspondence to: A.A. Reunov, Laboratory of Cell Differentiation, Paltchevsky st.-17, A.V. Zhirmunsky Institute of Marine Biology, Far East Branch of the Russian Academy of Sciences, 690041 Vladivostok, Russia. Tel: +7 4232 311143. Fax: +7 4232 310900. e-mail: arkadiy_reunov@hotmail.com
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Summary

Mouse Vasa homologue (MVH) antibodies were applied to mouse Graafian oocytes to clarify if mitochondrion-originated germinal body-like structures, described previously by conventional electron microscopy, were associated with the germ plasm. It was found that both the mitochondrion-like structures with cristae and the germinal body-like structures that lacked any signs of cristae were labelled specifically by the anti-MVH antibody. Moreover, some granules were MVH-positive ultrastructural hybrids of the mitochondria and germinal body-like structures, the presence of which clearly supported the idea of a mitochondrial origin for the germinal body-like structures. This finding is the first evidence that mitochondrion-originated germinal body-like granules represent mouse germ plasm.

Keywords

Germ plasmGerminal body-like structuresMouseMVH proteinOocytes

Information

Type
Research Article
Information
Zygote , Volume 23 , Issue 4 , August 2015 , pp. 501 - 506
Copyright
Copyright © Cambridge University Press 2014 

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