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Ultraviolet radiation and handling medium osmolarity affect chimaerism success in zebrafish

Published online by Cambridge University Press:  23 June 2010

M. Francisco-Simão ,
J. Cardona-Costa ,
M. Pérez-Camps  and
F. García-Ximénez
M. Francisco-Simão
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain. Faculty of Agriculture Sciences – Agostinho Neto University, Angola.
J. Cardona-Costa*
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain.
M. Pérez-Camps
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain.
F. García-Ximénez
Affiliation:
Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain.
*
All correspondence to: J. Cardona-Costa. Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain. Tel: +34 963879433. Fax: +34 963877439. e-mail: cardona_costa_j@hotmail.com
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Summary

The effects of a predefined ultraviolet radiation dose (0.529 mW/cm2 for 30s) together with two different micromanipulation medium osmolarities (30 mOsm/kg vs 300 mOsm/kg) were tested on embryo survival at different developmental stages and on the somatic (skin) and germ-line chimaerism rates. Somatic (13%, 6/47 adults) and germ-line chimaerism (50% pigmented F1 larvae) were detected only in the UV-treated recipient embryos micromanipulated in a 300 mOsm/kg medium. From the results obtained, we concluded that the conditions cited above were the most suitable to improve somatic and germ-line chimaerism rates in zebrafish.

Keywords

EmbryoGerm-line chimaerismOsmolarityZebrafish

Information

Type
Research Article
Information
Zygote , Volume 19 , Issue 1 , February 2011 , pp. 67 - 70
Copyright
Copyright © Cambridge University Press 2010

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