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Extender supplementation with catalase maintains the integrity of sperm plasma membrane after freezing–thawing of semen from capuchin monkey

Published online by Cambridge University Press:  09 March 2017

Danuza L. Leão ,
Adriel B. Brito ,
Stefânia A. Miranda ,
Karol G. Oliveira ,
Débora V.C. Almeida ,
Regiane R. Santos  and
Sheyla F. S. Domingues
Danuza L. Leão
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil.
Adriel B. Brito
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil.
Stefânia A. Miranda
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil.
Karol G. Oliveira
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil. National Primate Center, Ananindeua, PA, Brazil.
Débora V.C. Almeida
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil.
Regiane R. Santos*
Affiliation:
Federal University of Pará, Laboratory of Wild Animal Biology and Medicine, BR 316 Km 61, CEP 68740–970, Castanhal, Pará, Brazil
Sheyla F. S. Domingues
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil.
*
All correspondence to: Regiane R Santos. Federal University of Pará, Laboratory of Wild Animal Biology and Medicine, BR 316 Km 61, CEP 68740–970, Castanhal, Pará, Brazil. E-mail: R.Rodriguesdossantos@pq.cnpq.br
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Summary

We aimed to evaluate the effect of supplementation of ACP-118® extender with the antioxidant catalase (10 and 50 µg/ml) on Sapajus apella sperm motility, vigour, and plasma membrane integrity during the processes of seminal liquefaction, cooling, and freezing. Catalase did not affect any of the evaluated parameters after semen dilution or cooling. Cryopreserved sperm in the presence of 50 µg/ml catalase presented a plasma membrane integrity similar to that fresh sperm, however.

Keywords

AntioxidantCell membraneMonkeySemen

Information

Type
Research Article
Information
Zygote , Volume 25 , Issue 2 , April 2017 , pp. 231 - 234
Copyright
Copyright © Cambridge University Press 2017 

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