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K+-dependent Müller cell-generated components of the electroretinogram

Published online by Cambridge University Press:  23 July 2021

Andrey V. Dmitriev Open the ORCID record for Andrey V. Dmitriev [Opens in a new window] ,
Alexander A. Dmitriev  and
Robert A. Linsenmeier
Andrey V. Dmitriev*
Affiliation:
Department of Biomedical Engineering, Northwestern University, Evanston, Illinois
Alexander A. Dmitriev
Affiliation:
Department of Biomedical Engineering, Northwestern University, Evanston, Illinois
Robert A. Linsenmeier
Affiliation:
Department of Biomedical Engineering, Northwestern University, Evanston, Illinois Department of Neurobiology, Northwestern University, Evanston, Illinois Department of Ophthalmology, Northwestern University, Chicago, Illinois
*
Address correspondence to: *Andrey V. Dmitriev, Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd, #310, Evanston, IL 60208. E-mail: andrey.dmitriev@northwestern.edu
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Abstract

The electroretinogram (ERG) has been employed for years to collect information about retinal function and pathology. The usefulness of this noninvasive test depends on our understanding of the cell sources that generate the ERG. Important contributors to the ERG are glial Müller cells (MCs), which are capable of generating substantial transretinal potentials in response to light-induced changes in extracellular K+ concentration ([K+]o). For instance, the MCs generate the slow PIII (sPIII) component of the ERG as a reaction to a photoreceptor-induced [K+]o decrease in the subretinal space. Similarly, an increase of [K+]o related to activity of postreceptor retinal neurons also produces transretinal glial currents, which can potentially influence the amplitude and shape of the b-wave, one of the most frequently analyzed ERG components. Although it is well documented that the majority of the b-wave originates from On-bipolar cells, some contribution from MCs was suggested many years ago and has never been experimentally rejected. In this work, detailed information about light-evoked [K+]o changes in the isolated mouse retina was collected and then analyzed with a relatively simple linear electrical model of MCs. The results demonstrate that the cornea-positive potential generated by MCs is too small to contribute noticeably to the b-wave. The analysis also explains why MCs produce the large cornea-negative sPIII subcomponent of the ERG, but no substantial cornea-positive potential.

Keywords

Müller cellselectroretinogramretinaK+-selective microelectrodescomputer simulation

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Type
Research Article
Information
Visual Neuroscience , Volume 38 , 2021 , E010
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Copyright
© The Author(s), 2021. Published by Cambridge University Press

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