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Genotypic variation in callus induction and plant regeneration of Saccharum barberi

Published online by Cambridge University Press:  16 September 2025

Min Zhang ,
Jun Mao ,
Xin Lu ,
Xujuan Li ,
Chunyan Tian ,
Xin Hu ,
Chunjia Li  and
Luodong Huang Open the ORCID record for Luodong Huang [Opens in a new window]
Min Zhang
Affiliation:
College of Life Science and Technology, Guangxi University, Nanning, China National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Jun Mao
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Xin Lu
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Xujuan Li
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Chunyan Tian
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Xin Hu
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Chunjia Li*
Affiliation:
National Key Laboratory for Biological Breeding of Tropical Crops, Yunnan Academy of Agricultural Sciences, Kunming, YN, China Yunnan Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan, YN, China
Luodong Huang*
Affiliation:
College of Life Science and Technology, Guangxi University, Nanning, China
*
Corresponding author: Luodong Huang; Email: ynhuangld@gxu.edu.cn; Chunjia Li; Email: lcjzt@163.com
Corresponding author: Luodong Huang; Email: ynhuangld@gxu.edu.cn; Chunjia Li; Email: lcjzt@163.com
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Abstract

Saccharum barberi is regarded as a sugarcane germ plasm resource of potential value. Tissue culture serves multiple purposes in breeding-related research for sugarcane. The response to tissue culture varies considerably among sugarcane genotypes; however, the influence of genetic differences on the tissue culture performance of S. barberi had not been previously investigated. This study evaluated the genotypic variation in tissue culture response among six accessions of S. barberi. Seven parameters were assessed to determine the tissue culture performance: callus induction frequency (CIF), embryogenic callus ratio, embryogenic callus induction frequency, callus regeneration frequency, callus regeneration coefficient, overall regeneration frequency (ORF) and overall regeneration coefficient (ORC). Significant variations (P < 0.05) were observed among the S. barberi genotypes for all parameters. The broad-sense heritability ranged from 80.77% to 93.10%, indicating that genetic differences were the primary source of genotypic variation. ORF exhibited the highest diversity among the parameters, with a genotypic coefficient of variation up to 70.06%. Pansahi was identified as the most amenable genotype to tissue culture, demonstrating superior performance in both callus induction and plant regeneration. CIFs at different induction periods were strongly positively correlated with both ORF and ORC, particularly during the first week, suggesting that CIF may serve as a promising early predictor of overall regeneration competence. This study is the first to report the effect of genotypic variation on callus induction and plant regeneration of S. barberi, and the findings will be valuable for future research involving tissue culture in this species.

Keywords

callus inductiongenotypic variationplant regenerationSaccharum barberisugarcane

Information

Type
Research Article
Information
Plant Genetic Resources , First View , pp. 1 - 9
Copyright
© The Author(s), 2025. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany.

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