Hostname: page-component-cb9f654ff-c75p9 Total loading time: 0 Render date: 2025-09-03T11:35:42.520Z Has data issue: false hasContentIssue false
  • English
  • Français

Serological differentiation of the potato-cyst nematodes Globodera pallida and G. rostochiensis: partial purification of species-specific proteins

Published online by Cambridge University Press:  06 April 2009

A. Schots ,
J. Bakker ,
F. J. Gommers ,
L. Bouwman-Smits  and
E. Egberts
A. Schots
Affiliation:
Department of Nematology, Agricultural University, Binnenhaven 10, 6709 PD Wageningen, The Netherlands Department of Experimental Animal Morphology and Cell Biology, Agricultural University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands
J. Bakker
Affiliation:
Department of Nematology, Agricultural University, Binnenhaven 10, 6709 PD Wageningen, The Netherlands
F. J. Gommers
Affiliation:
Department of Nematology, Agricultural University, Binnenhaven 10, 6709 PD Wageningen, The Netherlands
L. Bouwman-Smits
Affiliation:
Department of Nematology, Agricultural University, Binnenhaven 10, 6709 PD Wageningen, The Netherlands
E. Egberts
Affiliation:
Department of Experimental Animal Morphology and Cell Biology, Agricultural University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands
Get access Rights & Permissions [Opens in a new window]

Summary

Two major groups of heat-stable proteins have been purified by heat denaturation from homogenates of eggs of the potato-cyst nematodes Globodera rostochiensis and G. pallida. SDS-polyacrylamide gel electrophoresis of protein homogenates from 6 G. rostochiensis populations and 7 G. pallida populations revealed 2 bands specific for G. rostochiensis and 3 bands specific for G. pallida. Two-dimensional electrophoresis showed that the 2 bands specific for G. rostochiensis consisted of 2 polypeptides differing slightly in isoelectric point, as did one of the bands specific for G. pallida. Conventional antisera made against protein homogenates of either Globodera species showed a complete cross-reaction with the species-specific proteins. The perspectives of the differences in protein composition between G. rostochiensis and G. pallida, established in this study, for a quantitative differentiation of mixed field populations of the two Globodera species, involving monoclonal antibodies, are discussed.

Information

Type
Research Article
Information
Parasitology , Volume 95 , Issue 2 , October 1987 , pp. 421 - 428
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Bakker, J. & Gommers, F. J. (1982). Differentiation of the potato cyst nematodes Globodera rostochiensis and G. pallida and of two G. rostochiensis pathotypes by means of two-dimensional electrophoresis. Proceedings of the Koninklijke Nederlandse Akademie van Wetenschappen C85, 309–14.Google Scholar
Bergé, J. B., Dalmasso, A., Person, F., Rivoal, R. & Thomas, D. (1981). Isoestérases chez le nématodes Heterodera avenae. I- Polymorphisme chez différentes races françhises. Revue de Nématologie 4, 99–105.Google Scholar
Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–54.CrossRefGoogle ScholarPubMed
Dalmasso, A. & Bergé, J. B. (1983). Enzyme polymorphism and the concept of parthenogenetic species, exemplified by Meloidogyne. In Concepts in Nematode Systematics. Systematics Association Special Vol. No. 22 (ed. Stone, A. R., Platt, H. M. and Khalil, L. K.), pp. 187–196. New York and London: Academic Press.Google Scholar
Fleming, C. C. & Marks, R. J. (1982). A method for the quantitative estimation of Globodera rostochiensis and Globodera pallida in mixed species samples. Record of Agricultural Research 30, 67–70.Google Scholar
Fleming, C. C. & Marks, R. J. (1983). The identification of the potato cyst nematodes Globodera rostochiensis and G. pallida by isoelectric focussing of proteins on polyacrvlamide gels. Annals of Applied Biology 103, 277–81.CrossRefGoogle Scholar
Fox, P. C. & Atkinson, H. J. (1984). Isoelectric focusing of general protein and specific enzymes from pathotypes of Globodera rostochiensis and G. pallida. Parasitology 88, 131–9.CrossRefGoogle Scholar
Franco, J. (1979). Disc electrophoresis of female proteins of British and Peruvian potato cyst nematode populations, Globodera spp. Nematologica 25, 32–5.CrossRefGoogle Scholar
Greet, D. N. & Firth, J. (1977). Influence of host plant on electrophoretic protein patterns of some round-cyst nematode females and use of larvae to obtain less ambiguous results. Nematologica. 23, 411–15.Google Scholar
Kort, J., Ross, H., Rumpenhorst, H. J. & Stone, A. R. (1978). An international scheme for identifying and classifying pathotypes of potato cyst-nematodes Globodera. rostochiensis and G. pallida. Nematologica 23, 333–9.CrossRefGoogle Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Misaghi, I. & McClure, M. A. (1974). Antigenic relationship of Meloidogyne incognita, M. javanica and M. arenaria. Phytopathology 64, 698–701.CrossRefGoogle Scholar
Oakley, B. R., Kirsch, D. R. & Morris, N. R. (1980). A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105, 361–3.CrossRefGoogle ScholarPubMed
O'Farrell, P. H. (1975). High resolution two dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 4007–21.CrossRefGoogle ScholarPubMed
Stegemann, H., Francksen, H. & Rumpenhorst, H. J. (1982). Differenzierung der Nematoden Globodera rostochiensis und G. pallida durch ein- und zweidimensionale Elektrophoresen ihrer Proteine. Nachrichtenblatt der Deutschen Pflanzenschutzdienst 34, 3–7.Google Scholar
Stone, A. R. (1973). Heterodera pallida n.sp. (Nematoda: Heteroderidae), a second species of potato cyst nematode. Nematologica 18, 591–606.CrossRefGoogle Scholar
Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4.CrossRefGoogle ScholarPubMed
Trudgill, D. L. & Carpenter, J. M. (1971). Disk electrophoresis of proteins of Heterodera species and pathotypes of Heterodera rostochiensis. Annals of Applied Biology 69, 35–41.CrossRefGoogle Scholar
Trudgill, D. L. & Parrott, D. M. (1972). Disc electrophoresis and larval dimensions of British, Dutch and other populations of Heterodera rostochiensis, as evidence of the existence of two species, each with pathotypes. Nematologica 18, 141–8.CrossRefGoogle Scholar
Webster, J. M. & Hooper, D. J. (1968). Serological and morphological studies on the inter- and intraspecific differences of the plant-parasitic nematodes Heterodera and Ditylenchus. Parasitology 58, 879–91.CrossRefGoogle Scholar
Wharton, R. J., Storey, R. M. J. & Fox, P. C. (1983). The potential of some immunochemical and biochemical approaches to the taxonomy of potato cyst nematodes. In Concepts in Nematode Systematics. Systematics Association Special Vol. No. 22 (ed. Stone, A. R., Platt, H. M. and Khalil, L. K.) pp. 235–248. New York and London: Academic Press.Google Scholar