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Genetic variation in the mitochondrial cytochrome c oxidase subunit 1 within Progamotaenia festiva (Cestoda: Anoplocephalidae) from macropodid marsupials

Published online by Cambridge University Press:  27 April 2007

I. BEVERIDGE ,
S. SHAMSI ,
M. HU ,
N. B. CHILTON  and
R. B. GASSER
I. BEVERIDGE*
Affiliation:
Department of Veterinary Science, The University of Melbourne, Veterinary Clinical Centre, 250 Princes Highway, Werribee, Victoria 3030, Australia
S. SHAMSI
Affiliation:
Department of Veterinary Science, The University of Melbourne, Veterinary Clinical Centre, 250 Princes Highway, Werribee, Victoria 3030, Australia
M. HU
Affiliation:
Department of Veterinary Science, The University of Melbourne, Veterinary Clinical Centre, 250 Princes Highway, Werribee, Victoria 3030, Australia
N. B. CHILTON
Affiliation:
Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, Saskatchewan S7N 5EZ, Canada
R. B. GASSER
Affiliation:
Department of Veterinary Science, The University of Melbourne, Veterinary Clinical Centre, 250 Princes Highway, Werribee, Victoria 3030, Australia
*
*Corresponding author: Department of Veterinary Science, The University of Melbourne, Veterinary Clinical Centre, 250 Princes Highway, Werribee, Victoria 3030, Australia. Fax: +61 3 97312366. E-mail: ibeve@unimelb.edu.au
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Summary

Genetic variation was examined in the anoplocephalid cestode Progamotaenia festiva, from Australian marsupials, in order to test the hypothesis that P. festiva, is a complex of sibling species and to assess the extent of host switching reported previously based on multilocus enzyme electrophoresis (MEE). Polymerase chain reaction (PCR)-based single-strand conformational polymorphism (SSCP) was used for the analysis of sequence variation in the cytochrome c oxidase subunit 1 (cox1) gene among 179 specimens of P. festiva (identified based on morphology and predilection site in the host) from 13 different host species, followed by selective DNA sequencing. Fifty-three distinct sequence types (haplotypes) representing all specimens were defined. Phylogenetic analyses of these sequence data (utilizing maximum parsimony and neighbour-joining methods) revealed 12 distinct clades. Other heterologous species, P. ewersi and P. macropodis, were used as outgroups and the remaining bile-duct inhabiting species, P. diaphana and P. effigia, were included in the analysis for comparative purposes. The latter 2 species were nested within the clades representing P. festiva. Most clades of P. festiva identified were restricted to a single host species; one clade primarily in Macropus robustus was also found in the related host species M. antilopinus in an area of host sympatry; another clade occurring primarily in M. robustus occurred also in additional kangaroo species, M. rufus and M. dorsalis. High levels of genetic divergence, the existence of distinct clades and their occurrence in sympatry provide support for the hypothesis that P. festiva represents a complex of numerous species, most of which, but not all, are host specific. Three distinct clades of cestodes were found within a single host, M. robustus, but there was no evidence of within-host speciation.

Keywords

Progamotaeniacestodesmarsupialsgenetic variationmitochondrial DNAcytochrome c oxidase subunit 1 gene

Information

Type
Research Article
Information
Parasitology , Volume 134 , Issue 10 , September 2007 , pp. 1465 - 1476
Copyright
Copyright © Cambridge University Press 2007

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