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Diagnostic accuracy of somatic and excretory−secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis

Published online by Cambridge University Press:  05 July 2021

William Henry Roldán Gonzáles Open the ORCID record for William Henry Roldán Gonzáles [Opens in a new window] ,
Dirce Mary Correia Lima Meisel ,
Fabiana Martins de Paula Open the ORCID record for Fabiana Martins de Paula [Opens in a new window]  and
Ronaldo Cesar Borges Gryschek
William Henry Roldán Gonzáles
Affiliation:
Laboratório de Imunopatologia da Esquistossomose (Laboratório de Investigação Médica, LIM-06) Hospital das Clínicas, Faculdade de Medicina Universidade de São Paulo, São Paulo, Brazil
Dirce Mary Correia Lima Meisel
Affiliation:
Laboratório de Imunopatologia da Esquistossomose (Laboratório de Investigação Médica, LIM-06) Hospital das Clínicas, Faculdade de Medicina Universidade de São Paulo, São Paulo, Brazil
Fabiana Martins de Paula*
Affiliation:
Laboratório de Imunopatologia da Esquistossomose (Laboratório de Investigação Médica, LIM-06) Hospital das Clínicas, Faculdade de Medicina Universidade de São Paulo, São Paulo, Brazil Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil
Ronaldo Cesar Borges Gryschek
Affiliation:
Laboratório de Imunopatologia da Esquistossomose (Laboratório de Investigação Médica, LIM-06) Hospital das Clínicas, Faculdade de Medicina Universidade de São Paulo, São Paulo, Brazil Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil
*
Author for correspondence: Fabiana Martins de Paula, E-mail: fabiana.paula@hc.fm.usp.br
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Abstract

To evaluate the diagnostic accuracy of three types of antigenic preparations from Strongyloides venezuelensis infective larvae for detection of serum IgG anti-Strongyloides antibodies by enzyme-linked immunosorbent assay (ELISA). Soluble somatic fractions (SSF) and membrane somatic fractions (MSF) and excretory−secretory (E/S) products from S. venezuelensis infective larvae were evaluated against 71 sera from individuals with strongyloidiasis, 105 sera from healthy individuals, and 84 sera from individuals with other helminth infections. Using an ELISA cut-off for 100% sensitivity, E/S products were 97.88% specific followed by MSF (93.12%) and then by SSF (85.2%). The occurrence of cross-reactivity with other helminths was 4.76% (4/84) with E/S products, 8.33% (7/84) with MSF, and 17.86% (15/84) with SSF. For a cut-off for 100% specificity, E/S products showed a sensitivity of 88.73% whereas MSF and SSF showed sensitivities of 59.15% and 53.52%, respectively. In conclusion, E/S products were the best antigenic option for the serodiagnosis of human strongyloidiasis.

Keywords

Antibodyantigencross-reactivityserodiagnosisStrongyloidesstrongyloidiasis

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Type
Research Article
Information
Parasitology , Volume 148 , Issue 12 , October 2021 , pp. 1522 - 1527
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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