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Porous Scaffolds Consisting of Collagen, Chondroitin Sulfate,and Hydroxyapatite with Enhanced Biodegradable Resistance for CartilageRegeneration

Published online by Cambridge University Press:  14 March 2011

H. Kaneda ,
T. Ikoma ,
T. Yoshioka ,
M. Nishi ,
R. Matsumoto ,
T. Uemura ,
J. S. Cross  and
J. Tanaka
H. Kaneda
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
T. Ikoma
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
T. Yoshioka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
M. Nishi
Affiliation:
Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
R. Matsumoto
Affiliation:
Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
T. Uemura
Affiliation:
Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
J. S. Cross
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
J. Tanaka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
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Abstract

Porous scaffolds of alkaline-soluble collagen including nanocompositeparticles of chondroitin sulfate and low crystalline hydroxyapatite forcartilage regeneration were fabricated by freeze-drying and thermaldehydration treatments; porous collagen scaffolds were also synthesized as areference. The scaffolds were cross-linked using glutaraldehyde (GA) vaportreatment in order to enhance biodegradable resistance. Microstructuralobservation with scanning electron microscope indicated that the scaffoldswith and without GA cross-linkage had open pores between 130 to 200 μm indiameter and well-interconnected pores of 10 to 30 μm even aftercross-linkage. In vitro biodegradable resistance tocollagenase was significantly enhanced by GA cross-linking of the scaffolds.All these results suggest that the GA cross-linked scaffolds consisting ofcollagen, chondroitin sulfate, and low crystalline hydroxyapatite havesuitable microporous structures and long-term biochemical stability forcartilage tissue engineering.

Keywords

biomaterialcompositetissue

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References

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