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Novel Electrospun Bicomponent Scaffolds for Bone TissueEngineering: Fabrication, Characterization and Sustained Release of GrowthFactor

Published online by Cambridge University Press:  01 March 2012

Chong Wang ,
Min Wang  and
Xiao-Yan Yuan
Chong Wang
Affiliation:
Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road Hong Kong
Min Wang*
Affiliation:
Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road Hong Kong
Xiao-Yan Yuan
Affiliation:
School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China
*
*Corresponding author: memwang@hku.hk
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Abstract

Electrospinning is a versatile technique for fabricating three-dimensional(3D) nanofibrous scaffolds and the scaffolds have been found to elicitdesirable cellular behavior for tissue regeneration because the nanofibrousstructures mimic the nanofibrous extracellular matrix (ECM) of biologicaltissues. From the material point of view, the ECM of bone is a nanofibrousnanocomposite consisting of an organic matrix (mainly collagen) andinorganic bone apatite nanoparticles. Therefore, for bone tissue engineeringscaffolds, it is natural to construct nanofibrous nanocomposites having abiodegradable polymer matrix and nanosized bioactive bioceramics. Ourprevious studies demonstrated: (1) electrospun nanocomposite fiber loadedwith calcium phosphate (Ca-P) were osteoconductive and could promoteosteoblastic cell proliferation and differentiation better than pure polymerfibers; (2) The controlled release of recombinant human bone morphogeneticprotein (rhBMP-2) from scaffolds provided the scaffolds with desiredosteoinductivity. In the current investigation, novel bicomponent scaffoldsfor bone tissue engineering were produced using our established dual-sourcedual-power electrospinning technique to achieve both osteoconductivity andosteoinductivity. In the bicomponent scaffolds, one fibrous component waselectrospun Ca-P/PLGA nanocomposite fibers and the other component wasemulsion electrospun PDLLA nanofibers incorporated with rhBMP-2. Throughelectrospinning optimization, both fibers were evenly distributed inbicomponent scaffolds. The mass ratio of rhBMP-2/PDLLA fibers to Ca-P/PLGAfibers in bicomponent scaffolds could be controlled using multiple syringes.The structure and morphology of mono- and bicomponent scaffolds wereexamined. The in vitro release of rhBMP-2 from mono- andbicomponent scaffolds showed different release amount but similar releaseprofile, exhibiting an initial burst release. Blending PDLLA withpolyethylene glycol (PEG) could reduce the initial burst release ofrhBMP-2.

Keywords

biomaterialcompositenanostructure

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Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1418: Symposium LL/MM – Gels and Biomedical Materials , 2012 , mrsf11-1418-mm04-08
Copyright
Copyright © Materials Research Society 2012

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References

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