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Active Polymer Nanoparticles: Delivery of Antibiotics

Published online by Cambridge University Press:  01 February 2011

Monica Rabinovich ,
Shankari N. Somayaji ,
Rajeev Raghavan Pillai ,
Michael C. Hudson ,
J. Kent Ellington ,
Michael Bosse ,
James Horton  and
Kenneth E. Gonsalves
Monica Rabinovich
Affiliation:
mmrabino@email.uncc.edu, University of North Carolina at Charlotte, Polymer Nanotechnology Lab.at Center for Optoelectronics and Dept. of Chemistry, 9201 University City Blv., 358 GRIGG Hall, Charlotte, NC, 28223, United States, 704-687-8290, 704-687-8241
Shankari N. Somayaji
Affiliation:
ssomayaj@uncc.edu, University of North Carolina at Charlotte, Department of Biology, 9201 University City Blvd., 257 Woodward Hall, Charlotte, NC, 28223, United States
Rajeev Raghavan Pillai
Affiliation:
rraghav1@uncc.edu, University of North Carolina at Charlotte, Polymer Nanotechnology Lab.at Center for Optoelectronics and Dept. of Chemistry, 9201 University City Blvd., 358 GRIGG Hall, Charlotte, NC, 28223, United States
Michael C. Hudson
Affiliation:
mchudson@email.uncc.edu, University of North Carolina at Charlotte, Department of Biology, 9201 University City Blvd., 257 Woodward Hall, Charlotte, NC, 28223, United States
J. Kent Ellington
Affiliation:
jke@carolina.rr.com, Carolinas Medical Center, Department of Orthopaedic Surgery, 1616 Scott Ave., Charlotte, NC, 28203, United States
Michael Bosse
Affiliation:
Michael.Bosse@carolinashealthcare.org, Carolinas Medical Center, Department of Orthopaedic Surgery, 1616 Scott Ave., Charlotte, NC, 28203, United States
James Horton
Affiliation:
James.Horton@carolinashealthcare.org, Carolinas Medical Center, Department of Internal Medicine, 1000 Blythe Blvd., Charlotte, NC, 28232, United States
Kenneth E. Gonsalves
Affiliation:
kegonsal@email.uncc.edu, University of North Carolina at Charlotte, Polymer Nanotechnology Lab.at Center for Optoelectronics and Dept. of Chemistry, 9201 University City Blvd., 358 GRIGG Hall, Charlotte, NC, 28223, United States
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Abstract

Antibiotic-encapsulated PLA and PLGA nanoparticles were prepared by the single emulsion-solvent evaporation technique.Different PLA and PLGA systems were prepared, varying the copolymer composition and the amount of the surfactant polyvinyl alcohol. Characterization and drug loading studies were performed by UV-Visible spectrophotometry, dynamic light scattering, and scanning electron microscopy (SEM).

Simultaneously, in order to model the diffusion of the nanoparticles within the osteoblast, QDs such as functionalized InGaP/ZnS and polymer encapsulated InGaP/ZnS nanoparticles were added to confluent cultures of primary mouse osteoblasts. Following PreFer fixation, cultures were examined via confocal microscopy. QDs were clearly visible within osteoblasts.

Keywords

nanoscalenanostructurepolymer

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