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Effects of Echinostoma caproni infection on the phospholipid and sphingolipid content of the intestinal mucosa of ICR mice

Published online by Cambridge University Press:  05 June 2009

B.K. Albrecht ,
B. Fried  and
J. Sherma
B.K. Albrecht
Affiliation:
Departments of Chemistry, Lafayette College, Easton, Pennsylvania 18042, USA
B. Fried*
Affiliation:
Departments of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
J. Sherma
Affiliation:
Departments of Chemistry, Lafayette College, Easton, Pennsylvania 18042, USA
*
*Author for correspondence. Fax: 610 250 6557 E-mail: friedb@lafayette.edu
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Abstract

High performance thin layer chromatography (HPTLC) was used to determine phospholipids and sphingolipids in the intestinal mucosa of ICR mice infected with Echinostoma caproni for two weeks. The major phospholipids detected in both infected and non-infected mucosa were phosphatidylcholine (PC) and phosphatidylethanolamine (PE). HPTLC-densitometric analysis showed that there was a significant decrease in the weight of both PC and PE in the intestinal mucosa of infected mice compared to that of the uninfected controls. Cerebrosides and sulphatides, but not sphingomyelin, were identified in the intestinal mucosa of both infected and uninfected hosts. There was an apparent increase in the cerebroside content of the mucosa of infected versus control mice. The pathobiochemical changes seen in the polar lipid content of infected hosts probably reflect the feeding and behavioural activities of E. caproni in the mouse intestine.

Information

Type
Research Note
Information
Journal of Helminthology , Volume 72 , Issue 4 , December 1998 , pp. 355 - 357
Copyright
Copyright © Cambridge University Press 1998

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References

Frazer, B., Reddy, A., Fried, B. & Sherma, J. (1997) Effects of diet on the lipid composition of Echinostoma caproni (Trematoda) in ICR mice. Parasitology Research 83, 642–645.CrossRefGoogle ScholarPubMed
Fried, B. & Huffman, J.E. (1996) The biology of the intestinal trematode Echinostoma caproni. Advances in Parasitology 38, 311–368.CrossRefGoogle ScholarPubMed
Gennaro, L.A., Fried, B. & Sherma, J. (1996) HPTLC determination of phospholipids in snail-conditioned water from Helisoma trivolvis and Biomphalaria glabrata. Journal of Planar Chromatography – Modern TLC 9, 379–381.Google Scholar
Horutz, K. & Fried, B. (1995) Effects of Echinostoma caproni infection on the neutral lipid content of the intestinal mucosa of ICR mice. International Journal for Parasitology 25, 653–655.CrossRefGoogle ScholarPubMed
Rivas, F.Sudati, J., Fried, B. & Sherma, J. (1998) HPTLC analysis of neutral lipids in the intestinal mucosa, serum, and liver of ICR mice infected with Echinostoma caproni and of worm excretory-secretory products. Journal of Planar Chromatography – Modern TLC 11, 50–53.Google Scholar