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Hydatid cyst derived molecules potentiate the protective effect of sulfasalazine in murine induced colitis: Role of FOXP3 T-regulatory cells in colonic tissues

Published online by Cambridge University Press:  17 September 2025

A.M. Darwish Open the ORCID record for A.M. Darwish [Opens in a new window] ,
D.S. Fadaly ,
A.S.S. Mohamed  and
N.I.H. Ibrahim
A.M. Darwish*
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt
D.S. Fadaly
Affiliation:
Department of Clinical Pharmacology, Faculty of Medicine, Zagazig University, Egypt
A.S.S. Mohamed
Affiliation:
Department of Public Health and Community, Faculty of Medicine, Zagazig University, Egypt
N.I.H. Ibrahim
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt
*
Corresponding author: A.M. Darwish; Email: Asmaadarwish10@gmail.com
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Abstract

Ulcerative colitis (UC), a chronic recurrent inflammation of the colonic mucosa, has become a global emergency. With the restricted response and significant side effects of traditional drugs, a search for alternatives is mandatory. The current study aimed to evaluate the therapeutic effects of hydatid cyst fluid (HCF) and laminated layer (HLL) derived crude extract of E. granulosus, combined with the standard medication sulfasalazine (SLZ), for treating acetic acid-induced colitis. Mice were divided into 7 groups. A colitis model using acetic acid (150 μl of 5% con.) intrarectally was induced. On the 8th day following colitis induction, the mice were sacrificed. We assessed the severity of colitis using survival rate, disease activity index, colon weight/length ratio, colon weight/bodyweight ratio, and gross and histopathological score of colon damage. Immunohistochemistry for Foxp3 T-reg expression in colonic tissues was done. The levels of inflammatory cytokines tumor necrosis factor-α and IL-10, oxidative stress marker malondialdehyde, and total antioxidant capacity in serum samples were assessed. Groups treated with a combination of SLZ+HCF and SLZ+HLL revealed a statistically significant improvement compared to the colitis group by improving disease activity index, colon weight/length ratio and colon weight/bodyweight ratio, and gross and histopathological colon damage scores. Additionally, there was a significant rise in IL-4, TAC, and Foxp3+ Treg cell expression and decreased TNF-α and MDA expression. E. granulosus-derived HCF and HLL potentiate the protective effect of SLZ by increasing anti-inflammatory and antioxidant properties against murine colitis, suggesting the potential for novel therapeutic approaches.

Keywords

IBDE. granulosusHCFSLZHLL

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Type
Research Paper
Information
Journal of Helminthology , Volume 99 , 2025 , e107
Copyright
© The Author(s), 2025. Published by Cambridge University Press

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