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Effect of bovine lactoferrin and lactoferrin-derived peptides on planktonic cells and abiotic surface biofilms of Salmonella enterica

Published online by Cambridge University Press:  18 August 2025

Uriel A. Angulo-Zamudio ,
Adrian Canizalez-Roman ,
Hector Flores-Villaseñor ,
Jan G.M. Bolscher ,
Kamran Nazmi ,
Claudia Leon-Sicairos ,
Erika Acosta-Smith ,
Laura E. Quintero-Martínez  and
Nidia León-Sicairos
Uriel A. Angulo-Zamudio
Affiliation:
School of Medicine, Autonomous University of Sinaloa, Culiacan, SI, Mexico
Adrian Canizalez-Roman
Affiliation:
School of Medicine, Autonomous University of Sinaloa, Culiacan, SI, Mexico The Women's Hospital, Secretariat of Health, Culiacan, SI, Mexico
Hector Flores-Villaseñor
Affiliation:
School of Medicine, Autonomous University of Sinaloa, Culiacan, SI, Mexico The Sinaloa State Public Health Laboratory, Secretariat of Health, Culiacan, SI, Mexico
Jan G.M. Bolscher
Affiliation:
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, Netherlands
Kamran Nazmi
Affiliation:
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, Netherlands
Claudia Leon-Sicairos
Affiliation:
Integral Postgraduate Program in Biotechnology, FCQB, Autonomous University of Sinaloa, Culiacan, SI, Mexico
Erika Acosta-Smith
Affiliation:
School of Medicine, Autonomous University of Sinaloa, Culiacan, SI, Mexico
Laura E. Quintero-Martínez
Affiliation:
Integral Postgraduate Program in Biotechnology, FCQB, Autonomous University of Sinaloa, Culiacan, SI, Mexico
Nidia León-Sicairos*
Affiliation:
School of Medicine, Autonomous University of Sinaloa, Culiacan, SI, Mexico Pediatric Hospital of Sinaloa, Culiacan, SI, Mexico
*
Corresponding author: Nidia León-Sicairos; Email: nidialeon@uas.edu.mx
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Abstract

Salmonella enterica is a Gram-negative bacterium responsible approximately for 155,000 deaths annually. S. enterica is one of the most important foodborne pathogens, affecting mainly people in developed countries. The human immune system produces antibacterial peptides and proteins like lactoferrin (LF). This work addresses the hypothesis that bovine lactoferrin (bLF) and its derivative peptides bLactoferricin17-30, bD-Lactoferricin17-30, bLactoferrampin265-284, bD-Lactoferrampin265-284 and bLF-chimera have antimicrobial activity on planktonic cells and pre-formed biofilms of S. enterica. Planktonic Salmonella enterica ATCC 14028 were treated with bLF and bLF-peptides for two hours, and bacterial viability was determined by counting colony-forming units/ml. In addition, S. enterica biofilms were pre-formed or established on an abiotic surface, and viability or disruption was assessed in the presence of bLF and bLF-peptides by counting colony-forming units/ml or using the live/dead viability kit. We observed that bLF and bLF-peptides were bactericidal against planktonic S. enterica, killing more than 80% of cultures after two hours of treatment. The bactericidal effect was concentration and time-dependent. In addition, bLF, bLFampin165-284, and bLF-chimera showed an anti-biofilm effect against Salmonella biofilms pre-formed during 8 and 12 hours on the abiotic surface, disorganizing more than 50% of the biofilms after 4 or 6 hours of treatment. We conclude that bLF and its peptides show antimicrobial activity against planktonic cells and pre-formed biofilms of S. enterica on abiotic surfaces and could potentially be a therapeutic solution to combat Salmonella infections.

Keywords

Biofilmsbovine lactoferrinLF chimeraLF peptidessalmonella

Information

Type
Research Article
Information
Journal of Dairy Research , Volume 92 , Issue 1 , February 2025 , pp. 89 - 97
Copyright
© The Author(s), 2025. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

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