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Culture and fertilization of sheep ovarian oocytes:IV. Observations on sperm penetration in oocytes transferred to the sheep oviduct

Published online by Cambridge University Press:  27 March 2009

T. F. Crosby ,
P. O. M. Ryan  and
I. Gordon
T. F. Crosby
Affiliation:
Faculty of Agriculture, University College, Dublin
P. O. M. Ryan
Affiliation:
Faculty of Agriculture, University College, Dublin
I. Gordon
Affiliation:
Faculty of Agriculture, University College, Dublin
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Summary

Transfer was made of 2958 sheep ovarian oocytes, cultured in growth medium for 24–30 h, to the oviducts of 142 oestrous sheep; 132 were bred and ten remained unbred. Forty-two per cent of oocytes were recovered, mainly 4–24 h after transfer; tubal flushings were examined for sperm. Evidence of a sperm within the vitellus of follicular oocytes was found in nine recipients, the earliest at 6 h 50 min after transfer. Pronucleate eggs were most frequent 9–14 h after transfer; a limited number of eggs showing normal cleavage wasrecovered.

Information

Type
Research Article
Information
The Journal of Agricultural Science , Volume 76 , Issue 3 , June 1971 , pp. 379 - 382
Copyright
Copyright © Cambridge University Press 1971

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References

REFERENCES

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