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Nucleic acids in mummified plant seeds: biochemistry and molecular genetics of pre-Columbian maize

Published online by Cambridge University Press:  14 April 2009

Franco Rollo ,
Franco Maria Venanzi  and
Augusto Amici
Franco Rollo*
Affiliation:
Dipartimento di Biologia Molecolare, Cellulare e Animale, Università di Camerino, via F. Camerini 2, 1–62032 Camerino, Italy
Franco Maria Venanzi
Affiliation:
Dipartimento di Biologia Molecolare, Cellulare e Animale, Università di Camerino, via F. Camerini 2, 1–62032 Camerino, Italy
Augusto Amici
Affiliation:
Dipartimento di Biologia Molecolare, Cellulare e Animale, Università di Camerino, via F. Camerini 2, 1–62032 Camerino, Italy
*
* To whom correspondence should be sent.
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Nucleic acids fractions were isolated from pre-Columbian maize seeds and characterized using different approaches such as polyacrylamide gel electrophoresis, anti-DNA antibody binding, HPLC fractionation, molecular hybridization with cloned genes, and DNA amplification by the polymerase chain reaction. The nucleic acids were found to be very depolymerized (≤140 base pairs in length) and composed mainly of ribosomal RNA. Despite the very low amount and degree of polymerization of seed DNA, specific maize nuclear Mul, Mu4, Mu8 and, possibly, Mu5 element components could be detected, thanks to the use of amplification systems as short as 90 bp. The results suggest that evaluation of the relative proportions of Mu-type element components and, possibly, other maize genomic components in single mummified kernels, may offer a new key to the study of ancient maize populations.

Information

Type
Research Article
Information
Genetics Research , Volume 58 , Issue 3 , December 1991 , pp. 193 - 201
Copyright
Copyright © Cambridge University Press 1991

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