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Application of multiplex immunoassay technology to investigations of ocular disease

Published online by Cambridge University Press:  31 August 2016

Valerie Sloane Jones ,
Jian Wu ,
Si-Wei Zhu  and
Ruo-Pan Huang
Valerie Sloane Jones
Affiliation:
Raybiotech, Inc., Norcross, GA, USA
Jian Wu
Affiliation:
The Affiliated third Hospital of Sun Yat-sen University, Sun Yat-sen University, Guangdong, China
Si-Wei Zhu
Affiliation:
RayBiotech, Inc., Guangzhou, China
Ruo-Pan Huang*
Affiliation:
Raybiotech, Inc., Norcross, GA, USA RayBiotech, Inc., Guangzhou, China South China Biochip Research Center, Guangzhou, China
*
*Corresponding author: Ruo-Pan Huang, Raybiotech, Inc, 3607 Parkway Lane, Suite 100, Norcross, GA 30092, USA. E-mail: rhuang@raybiotech.com
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Abstract

Eye-derived fluids, including tears, aqueous humour and vitreous humour often contain molecular signatures of ocular disease states. These signatures can be composed of cytokines, chemokines, growth factors, proteases and soluble receptors. However, the small quantities (<10 µl) of these fluids severely limit the detection of these proteins by traditional enzyme-linked immunosorbent assay or Western blot. To maximise the amount of information generated from the analysis of these specimens, many researchers have employed multiplex immunoassay technologies for profiling the expression or modification of multiple proteins from minute sample volumes.

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Review
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Expert Reviews in Molecular Medicine , Volume 18 , 2016 , e15
Copyright
Copyright © Cambridge University Press 2016 

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