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Immunoregulatory and neuroprotective activity of ovocystatin

Published online by Cambridge University Press:  01 September 2022

B. Stańczykiewicz ,
J. Gburek ,
K. Gołąb ,
B. Konopska  and
A. Zabłocka
B. Stańczykiewicz*
Affiliation:
Wroclaw Medical University, Department Of Psychiatry, Wroclaw, Poland
J. Gburek
Affiliation:
Wroclaw Medical University, Department Of Pharmaceutical Biochemistry, Wroclaw, Poland
K. Gołąb
Affiliation:
Wroclaw Medical University, Department Of Pharmaceutical Biochemistry, Wroclaw, Poland
B. Konopska
Affiliation:
Wroclaw Medical University, Department Of Pharmaceutical Biochemistry, Wroclaw, Poland
A. Zabłocka
Affiliation:
Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Department Of Microbiology, Wroclaw, Poland
*
*Corresponding author.

Abstract

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Introduction

Ovocystatin has beneficial properties for cognitive function in young rats and might prevents aging-related cognitive impairment in older animals, as well as reduces memory decline in APP/PS1 mice model.

Objectives

Our study aimed at assessing the impact of ovocystatin on microglia activation and neurogenesis.

Methods

Immunoactivation: Mouse wild type microglia were stimulated with ovocystatin at dose of 100 micrograms/ml. The effect of ovocystatin on nitric oxide production and interleukin 1 beta secretion were determined. Neurogenesis: Primary rat hippocampal neurons of H19-7 cell line was used. The impact of ovocystatin on proliferation, nitric oxide production, and expression of markers of neurogenesis: microtubule-associated protein 2 (MAP2, isoforms A/B and C/D) and Synapsin 1, were determined.

Results

It was shown that ovocystatin does not stimulate microglial cells to produce inflammatory mediators. Whereas, no toxic effect of ovocystatin (1-100 ug/ml) on H19-7 cells viability, and dose-dependent down-regulation of proliferation were demonstrated. It was also shown that in primary hippocampal neurons of H19-7 cells incubated with ovocystatin (100 micrograms/ml), the expression level of MAP2 C/D (75kDa) - characteristic form of immature neurons is unchanged. However, the increased expression of MAP2 A/B protein (280 kDa) – characteristic for mature neurons was observed after 6 and 24h incubation with ovocystatin. Relatively to MAP2 A/B, increased expression of synapsin 1 was observed.

Conclusions

The ovocystatin might be a potential activator of molecular mechanisms in primary hippocampal neurons, participating in regulation of neurogenesis. Nevertheless, further studies are needed.

Disclosure

No significant relationships.

Keywords

ovocystatinImmunoactivationNeurogenesis

Information

Type
Abstract
Information
European Psychiatry , Volume 65 , Special Issue S1: Abstracts of the 30th European Congress of Psychiatry , June 2022 , pp. S700
Creative Commons
Creative Common License - CCCreative Common License - BY
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright
© The Author(s), 2022. Published by Cambridge University Press on behalf of the European Psychiatric Association
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