In this study, we assessed behavioral responses to social separation at 8 months of age andcerebrospinal fluid (CSF) concentrations of biogenic amines and metabolites at 8 and 18 monthsof age in 12 rhesus monkeys derived from either stressed or undisturbed pregnancies. Comparedto controls from undisturbed pregnancies, prenatal stress-derived monkeys had higherconcentrations of 3-methoxy-4-hydroxyphenylglycol (MHPG), and 3,4-dihydroxyphenylaceticacid in CSF than controls. Norepinephrine and MHPG response to stress were both correlatedbetween 8 and 18 months of age. There were few group differences in behavior during socialseparation; however, several behavioral differences between groups were found when monkeyswere reunited with cage mates. Prenatally stressed monkeys spent more time clinging to theirsurrogates and exploring (including eating and drinking), while controls showed morelocomotion and social play with their cage mates. Collectively, our findings suggest that chronicunpredictable psychological stress during pregnancy has long-lasting effects on noradrenergicand dopaminergic activity and behavior in the offspring of gestationally stressed primatemothers.