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GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE): IV. ELECTROPHORETIC BANDING PATTERNS OF OCTANOL DEHYDROGENASE AND ARGININE PHOSPHOKINASE

Published online by Cambridge University Press:  31 May 2012

R.H. Gooding  and
B.M. Rolseth
R.H. Gooding
Affiliation:
Department of Entomology, University of Alberta, Edmonton, Alberta T6G 2E3
B.M. Rolseth
Affiliation:
Department of Entomology, University of Alberta, Edmonton, Alberta T6G 2E3
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Abstract

Polyacrylamide gel electrophoresis of the thoraces of adult Glossina morsitans morsitans Westwood revealed five octanol dehydrogenase (ODH, E.C. 1.1.1.73) phenotypes (and a sixth was predicted) in males and females, two arginine phosphokinase (APK, E.C. 2.7.3.3) phenotypes in males and three APK phenotypes in females. Each enzyme was postulated to be under the control of a single locus; Odh with three alleles on an autosome and Apk with two alleles on the X-chromosome. Allele frequencies were in Hardy-Weinberg equilibrium in our colony, and breeding experiments provided direct evidence for single locus control of each enzyme. The polyacrylamide gel electrophoresis technique described permits determination of banding patterns for xanthine oxidase, aldehyde oxidase, ODH, and APK from a single individual.

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Type
Articles
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The Canadian Entomologist , Volume 111 , Issue 11 , November 1979 , pp. 1307 - 1310
Copyright
Copyright © Entomological Society of Canada 1979

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