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Distribution of [3H]trans-resveratrol in rat tissues following oral administration

Published online by Cambridge University Press:  08 March 2007

Manal Abd El-Mohsen ,
Henry Bayele ,
Gunter Kuhnle ,
Glenn Gibson ,
Edward Debnam ,
S. Kaila Srai ,
Catherine Rice-Evans  and
Jeremy P. E. Spencer
Manal Abd El-Mohsen
Affiliation:
School of Food Biosciences, University of Reading, PO Box 226, Whiteknights, Reading RG6 6AP, UK
Henry Bayele
Affiliation:
Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
Gunter Kuhnle
Affiliation:
Wolfson Centre for Age-related Diseases, GKT School of Biomedical Sciences, King's College, London SE1 9RT, UK
Glenn Gibson
Affiliation:
School of Food Biosciences, University of Reading, PO Box 226, Whiteknights, Reading RG6 6AP, UK
Edward Debnam
Affiliation:
Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
S. Kaila Srai
Affiliation:
Royal Free and University College Medical School, Royal Free Campus, London NW3 2PF, UK
Catherine Rice-Evans
Affiliation:
Wolfson Centre for Age-related Diseases, GKT School of Biomedical Sciences, King's College, London SE1 9RT, UK
Jeremy P. E. Spencer*
Affiliation:
School of Food Biosciences, University of Reading, PO Box 226, Whiteknights, Reading RG6 6AP, UK
*
*Corresponding author: Dr Jeremy P. E. Spencer, fax +44 (0)118 9310080, email j.p.e.spencer@reading.ac.uk
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Abstract

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Resveratrol has been widely investigated for its potential health properties, although little is known about its metabolism in vivo Here we investigated the distribution of metabolic products of [3H]trans-resveratrol, following gastric administration. At 2h, plasma concentrations reached 1·7% of the administered dose, whilst liver and kidney concentrations achieved 1·0 and 0·6%, respectively. Concentrations detected at 18h were lower, being only 0·5% in plasma and a total of 0·35% in tissues. Furthermore, whilst kidney and liver concentrations fell to 10 and 25%, respectively, of concentrations at 2h, the brain retained 43% of that measured at 2h. Resveratrol-glucuronide was identified as the major metabolite, reaching 7μm in plasma at 2h. However, at 18h the main form identified in liver, heart, lung and brain was native resveratrol aglycone, indicating that it is the main form retained in the tissues. No phenolic degradation products were detected in urine or tissues, indicating that, unlike flavonoids, resveratrol does not appear to serve as a substrate for colonic microflora. The present study provides additional information about the nature of resveratrol metabolites and which forms might be responsible for its in vivo biological effects.

Keywords

ResveratrolMetabolismPolyphenolRadioactiveDistribution

Information

Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 1 , January 2006 , pp. 62 - 70
Copyright
Copyright © The Nutrition Society 2006

References

Abd El-Mohsen, MM, Kuhnle, G, Moore, K, Debnam, E, Srai, S, Rice Evans, C & Spencer, JPAbsorption, tissue distribution and excretion of pelargonidin and its metabolites following oral administration to rats. Br J Nutr (2005) 94, 1–9.Google Scholar
Abd El-Mohsen, MM, Marks, J, Kuhnle, G, Rice-Evans, C, Moore, K, Gibson, G, Debnam, E & Srai, SKThe differential tissue distribution of the citrus flavanone naringenin following gastric instillation. Free Radic Res (2004) 38, 1329–1340.Google Scholar
Bravo, L, Abia, R, Eastwood, MA & Saura-Calixto, FDegradation of polyphenols (catechin and tannic acid) in the rat intestinal tract. Effect on colonic fermentation and faecal output. Br J Nutr (1994) 71, 933–946.CrossRefGoogle ScholarPubMed
Casper, RF, Quesne, M, Rogers, IM, Shirota, T, Jolivet, A, Milgrom, E & Savouret, JFResveratrol has antagonist activity on the aryl hydrocarbon receptor: implications for prevention of dioxin toxicity. Mol Pharmacol (1999) 56, 784–790.Google ScholarPubMed
Fauconneau, B, Waffo-Teguo, P, Huguet, F, Barrier, L, Decendit, A & Merillon, JMComparative study of radical scavenger and antioxidant properties of phenolic compounds from Vitis vinifera cell cultures using in vitro tests. Life Sci (1997) 61, 2103–2110.CrossRefGoogle ScholarPubMed
Goldberg, DM, Yan, J & Soleas, GJAbsorption of three winerelated polyphenols in three different matrices by healthy subjects. Clin Biochem (2003) 36, 79–87.CrossRefGoogle ScholarPubMed
Gross, M, Pfeiffer, M, Martini, M, Campbell, D, Slavin, J & Potter, JThe quantitation of metabolites of quercetin flavonols in human urine. Cancer Epidemiol Biomarkers Prev (1996) 5, 711–720.Google ScholarPubMed
Jager, U & Nguyen-Duong, HRelaxant effect of trans-resveratrol on isolated porcine coronary arteries. Arzneimittelforschung (1999) 49, 207–211.Google ScholarPubMed
Jang, M, Cai, L, Udeani, GO, Slowing, KV, Thomas, CF, Beecher, CW, Fong, HH, Farnsworth, NR, Kinghorn, AD, Mehta, RG, Moon, RC & Pezzuto, JMCancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science (1997) 275, 218–220.CrossRefGoogle ScholarPubMed
Kimura, Y, Okuda, H & Arichi, SEffects of stilbene derivatives on arachidonate metabolism in leukocytes. Biochim Biophys Acta (1985) 837, 209–212.Google ScholarPubMed
Lu, R & Serrero, GResveratrol, a natural product derived from grape, exhibits antiestrogenic activity and inhibits the growth of human breast cancer cells. J Cell Physiol (1999) 179, 297–304.3.0.CO;2-P>CrossRefGoogle ScholarPubMed
Marier, JF, Vachon, P, Gritsas, A, Zhang, J, Moreau, JP & Ducharme, MPMetabolism and disposition of resveratrol in rats: extent of absorption, glucuronidation, and enterohepatic recirculation evidenced by a linked-rat model. J Pharmacol Exp Ther (2002) 302, 369–373.Google Scholar
Rechner, AR, Kuhnle, G, Bremner, P, Hubbard, GP, Moore, KP & Rice-Evans, CAThe metabolic fate of dietary polyphenols in humans. Free Radic Biol Med (2002) 33, 220–235.Google Scholar
Rechner, AR, Pannala, AS & Rice-Evans, CACaffeic acid derivatives in artichoke extract are metabolised to phenolic acids in vivo. Free Radic Res (2001) 35, 195–202.CrossRefGoogle ScholarPubMed
Scheline, RRCRC Handbook of Mammalian Metabolism of Plant Compounds. Boca Raton, FL:. CRC Press. 1991.Google Scholar
Siemann, EH & Creasy, LLConcentration of the phytoaexlin resvertarol in red wine. Am J Enol Viticult (1992) 43, 49–52.Google Scholar
Soleas, GJ, Angelini, M, Grass, L, Diamandis, EP & Goldberg, DM 2001 Absorption of trans-resveratrol in rats. In Methods in Enzymology Flavonoids and Other Polyphenols. San Diego:. Academic Press. vol 335 Flavonoids and Other Polyphenols.Google Scholar
Soleas, GJ, Diamandis, EP, Goldberg, DMResveratrol: a molecule whose time has come?And gone?. Clin Biochem (1997) 30, 91–113.CrossRefGoogle ScholarPubMed
Spencer, JP, Chowrimootoo, G, Choudhury, R, Debnam, ES, Srai, SK & Rice-Evans, CThe small intestine can both absorb and glucuronidate luminal flavonoids. FEBS Lett (1999) 458, 224–230.Google Scholar
Trela, BC & Waterhouse, ALResveratrol: isomeric molar absorptivities and stability. J Agric Food Chem (1996) 44, 1253.CrossRefGoogle Scholar
Vitaglione, P, Sforza, S, Galaverna, G, Ghidini, C, Caporaso, N, Vescovi, PP, Fogliano, V & Marchelli, RBioavailability of trans-resveratrol from red wine in humans. Mol Nutr Food Res (2005) 49, 495–504.CrossRefGoogle ScholarPubMed
Vitrac, X, Desmouliere, A, Brouillaud, B, Krisa, S, Deffieux, G, Barthe, N, Rosenbaum, J & Merillon, JMDistribution of [14C]-trans-resveratrol, a cancer chemopreventive polyphenol, in mouse tissues after oral administration. Life Sci (2003) 72, 2219–2233.CrossRefGoogle ScholarPubMed
Walle, T, Hsieh, F, DeLegge, MH, Oatis, JE Jr & Walle, UKHigh absorption but very low bioavailability of oral resveratrol in humans. Drug Metab DisposEpublication 27 August 20042004 32, 1377–1382.Google Scholar
Wang, LX, Heredia, A, Song, HJ, Zhang, Z, Yu, B, Davis, C & Redfield, RResveratrol glucuronides as the metabolites of resveratrol in humans: characterization, synthesis, and anti-HIV activity. J Pharm Sci (2004) 93, 2448–2457.CrossRefGoogle ScholarPubMed
Wenzel, E, Soldo, T, Erbersdobler, H & Somoza, VBioactivity and metabolism of trans-resveratrol orally administered to Wistar rats. Mol Nutr Food Res (2005) 49, 482–494.CrossRefGoogle ScholarPubMed
Yu, C, Shin, YG, Chow, A, Li, YM, Kosmeder, JW, Lee, YS, Hirschelman, WH, Pezzuto, JM, Mehta, RG & von Breemen, RBHuman, rat, and mouse metabolism of resveratrol. Pharm Res (2002) 19, 1907–1914.Google Scholar