Hostname: page-component-cb9f654ff-5jtmz Total loading time: 0 Render date: 2025-09-02T04:46:47.180Z Has data issue: false hasContentIssue false
  • English
  • Français

Biochemical responses of broiler chicks to folate deficiency

Published online by Cambridge University Press:  09 March 2007

J. Sarah Rennie ,
Colin C. Whitehead  and
John Armstrong
J. Sarah Rennie
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian EH25 9PS
Colin C. Whitehead
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian EH25 9PS
John Armstrong
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian EH25 9PS
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effects of folate sufficiency and deficiency in three pathways of folate metabolism were studied in 2- and 3-week-old broiler chicks. Erythrocyte phosphoribosylpyrophosphate concentrations and dihydro- folate reductase (EC 1.5.1.3) activity were significantly elevated in folate deficiency. Percentage incorporation of deoxyuridine into bone marrow DNA was reduced in folate deficiency. There was a trend towards reduced liver dihydrofolate reductase activity in deficient chicks. These studies identify further biochemical criteria that can be used to assess folate status of chicks.

Keywords

Folic acidDihydrofolate reductaseDeoxyuridine suppressionPhosphoribosylpyrophosphateChicken

Information

Type
Vitamin Metabolism
Information
British Journal of Nutrition , Volume 69 , Issue 3 , May 1993 , pp. 801 - 808
Copyright
Copyright © The Nutrition Society 1993

References

Brody, T., Shane, B. & Stokstad, E. L. R. (1984). Folic acid. In Handbook of Vitamins, pp. 459–496 [Machlin, L. J., editor]. New York: Marcel Dekker.Google Scholar
Burns, R. A. & Jackson, N. (1976). Effects of folate deficiency on some hepatic enzymes of folate metabolism in the oestrogen-treated female chick (domestic fowl). Biochemical Society Transactions 4, 914–917.CrossRefGoogle ScholarPubMed
Cossins, E. A. (1984). Folates in biological materials. In Folates and Pterins, vol. 1, pp. 1–59 [Blakely, R. L. and Benkovic, S. J., editors]. New York: J. Wiley and Sons, Inc.Google Scholar
Deacon, R., Chanarin, I., Percy, J. & Lumb, M. (1981). The effect of folate analogues on thymidine utilisation by human and rat marrow cells and the effect on the deoxyuridine suppression test. Postgraduare Medical Journal 57, 611–616.CrossRefGoogle ScholarPubMed
Ghitis, J., Schreiber, C. & Waxman, S. (1987). Phosphate-induced phosphoribosylpyrophosphate elevations to assess deranged folate and purine nucleotide metabolism. Proceedings of the Society for Experimental Biology and Medicine 186, 90–95.CrossRefGoogle ScholarPubMed
Ghitis, J. & Waxman, S. (1987). Modified phosphoribosylpyrophosphate (PRPP) radioenzymatic assay: increased sensitivity, technical simplification and new applications. Clinica Chimica Acta 164, 267–273.CrossRefGoogle ScholarPubMed
Hershko, A., Razin, A. & Mager, J. (1969). Regulation of the synthesis of 5-phosphoribosyl-I-pyrophosphate in intact red blood cells and in cell-free preparations. Biochimica et Biophysica Acta 184, 64–76.CrossRefGoogle ScholarPubMed
Metz, J. (1984). The deoxyuridine suppression test. CRC Critical Reviews in Clinical Laboratory Sciences 20, 205–241.CrossRefGoogle ScholarPubMed
Metz, J., Kelly, A., Swett, V. C. & Waxman, S. (1968). Deranged DNA synthesis by bone marrow from vitamin B12 deficient humans. British Journal of Haematology 14, 575–592.CrossRefGoogle ScholarPubMed
National Research Council (1984). Nutrient Requirements of Poultry, 8th ed. Washington, DC: National Academy Press.Google Scholar
Whitehead, C. C. & Rennie, J. S. (1989). Relation between some folate-dependent metabolic pathways and dietary folate content in chicks. British Journal of Nutrition 62, 203–209.CrossRefGoogle ScholarPubMed
Wickramasinghe, S. N. & Longland, J. E. (1974). Assessment of deoxyuridine suppression test in diagnosis of B, or folate deficiency. British Medical Journal 3, 148–150.CrossRefGoogle ScholarPubMed
Wong, P. C.-K., Vohra, P. & Kratzer, F. H. (1977). The folate requirements of broiler chicks and quail (Coturnix coturnix japonica). Poultry Science 56, 1852–1860.CrossRefGoogle ScholarPubMed